Up-regulation of ferritin ubiquitination in skeletal muscle of transgenic rats bearing the G93A hmSOD1 gene mutation

A. Halon , A. Sielicka-Dudzin , M. Woźniak , W. Ziółkowski , W. Nyka , M. Herbik , P. Grieb , A. Figarski , Jędrzej Antosiewicz

Abstract

In the present study we measured the levels of protein carbolnyls and the H and L subunits of ferritin in three hind limb muscles, [Extensor digitorum longus, Tibialis anterior and Soleus] of transgenic rats bearing the G93A hmSOD1 gene and of their non-transgenic littermates. All of the muscles from the transgenic animals showed significantly higher protein carbonyl levels, compared to the respective muscles from control non-transgenic animals. In two muscles (Tibialis anterior and Soleus) from transgenic rats, both L and H subunits of ferritin were upregulated. Moreover, we observed that the electrophoretic mobility of both ferritin subunits was retarded which indicates their post-translational modification. Ferritin immunoprecipitation experiments show an increased ubiquitination of both H and L ferritin in all muscles from the transgenic animals. Our data show for the first time that ferritin ubiquitination could be responsible for oxidative stress in muscles of rats bearing the G93A hmSOD1, consequently ferritin is not able to control the labile iron pool.
Author A. Halon
A. Halon,,
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, A. Sielicka-Dudzin
A. Sielicka-Dudzin,,
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, M. Woźniak
M. Woźniak,,
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, W. Ziółkowski
W. Ziółkowski,,
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, W. Nyka
W. Nyka,,
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, M. Herbik
M. Herbik,,
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, P. Grieb
P. Grieb,,
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, A. Figarski
A. Figarski,,
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, Jędrzej Antosiewicz (Wydział Psychologii)
Jędrzej Antosiewicz,,
- Wydział Psychologii
Journal seriesNeuromuscular Disorders, ISSN 0960-8966, (A 30 pkt)
Issue year2010
Vol20
Pages29-33
Publication size in sheets0
Keywords in EnglishIron, Ubiquitin, Oxidative stress
DOIDOI:10.1016/j.nmd.2009.08.014
Languageen angielski
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Ha-on-NMD2157-1-.pdf of 14-09-2015
567.35 KB
Score (nominal)32
Citation count*
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Dorobek Naukowy - Preview URLhttp://dn.swps.edu.pl/Podglad.aspx?WpisID=1982
Dorobek Naukowy - Approve URLhttp://dn.swps.edu.pl/Biuro/ZatwierdzanieWpisu.aspx?WpisID=1982
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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